Activation of Peripheral Blood T Cells Via
نویسندگان
چکیده
IL-2 exerts multiple biological activities upon binding to its specific cell surface receptors. The IL-2 receptor (IL-2R) is composed of at least two subunits, the p55 (CD25/Tac) and the p75 glycoproteins. p55 and p75 both bind IL-2 independently with either low or intermediate affinity, respectively, whereas a heterodimeric receptor composed of p55 and p75 binds IL-2 with high affinity (1, 2) . p75 but not p55 has been suggested to be responsible for signal transduction (1, 2) . HumanPBLare readily activated by IL-2 to exhibit augmented cytotoxicity against NK-sensitive and NK-resistant target cells, which is known as the lymphokine-activated killer (LAK) phenomenon (3). CD3-CD16 + NK cells are the predominant LAK precursor and effector cells (4) . Recently, Phillips et al . (5) have directly assessed the involvement of the p75 IL-2R in the activation of peripheral blood NK cells by using a recently developed mAb, termed TU27, which specifically blocks IL-2 binding to the p75 IL-2R (6) . Although TU27 mAb preferentially reacts with CD3-CD16* NK cells (5), we have also demonstrated that peripheral blood CD8' but not CD4+ T cells exhibit a substantial reactivity with TU27 mAb, as estimated by flow cytometry (7) . In addition, several reports have described the direct activation of resting T cells by IL-2, and again the p75 IL-2R has been implicated in this response (8-10) . In the present study we have examined the role of p75 and p55 IL-2R in rapidly inducing peripheral blood T cell zytolytic activity by using the mAbs abrogating IL-2 binding to these subunits .
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